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1.
Southeast Asian J Trop Med Public Health ; 2004 Dec; 35(4): 817-9
Article in English | IMSEAR | ID: sea-33884

ABSTRACT

A 72 year old Thai male; a resident of Kapoe district, Ranong Province, Southern Thailand presented with conjunctivitis of the left eye. A filariid nematode was recovered from under the medial side of ocular conjunctiva of the left eye. The filariid nematode was identified as a young adult female Parafilaria bovicola, based on the following characteristics: no alteration of the transverse striations on the anterior end of the body, the terminal anus and the vulva opening is just posterior to the mouth. This is a first case report in Thailand of P bovicola causing human disease. P. bovicola has caused damage to the meat industry in many countries and has caused disease in humans. Further study of the life cycle, occurrence among cattle, and transmission of the disease between it's natural host and humans is necessary.


Subject(s)
Aged , Animals , Conjunctivitis/parasitology , Female , Humans , Male , Nematoda/anatomy & histology , Thailand
2.
Article in English | IMSEAR | ID: sea-137123

ABSTRACT

A case of bronchial infection with Mammomonogamus laryngeus in a 17-year-old man in Central Thailand is described. Following presentation with chronic unproductive cough, haemoptysis and weight loss, two pairs of worms were expelled. Treatment with albendazole resulted in resolution of the symptoms. Some aspects of Mammomonogamus laryngeus infection related to human parasitism, diagnosyic and the approach, treatment, and prophylaxis are also discussed.

3.
Southeast Asian J Trop Med Public Health ; 2003 Mar; 34(1): 1-6
Article in English | IMSEAR | ID: sea-33088

ABSTRACT

An in-house membrane dot-blot enzyme-linked immunosorbent assay kit, for the detection of specific antibody to a 31 kDa protein of Parastrongylus cantonensis was evaluated in a blinded multi-laboratory study. The kit was provided to technologists, who were trained in its use, from nine regional laboratories of Thailand. With an identical set of 20 coded serum samples and reference positive and negative controls, the kit was found to have a diagnostic sensitivity of 100% and a diagnostic specificity of 100% in all the laboratories. There was no obvious variation in quality among five lots of the antigen-coated nitrocellulose strips evaluated. The shelf life of the kit was > or = 6 months when the test components were stored at 4 degrees C and 18 months at -20 degrees C. The enzyme-linked immunoassay dot technique is easy to perform and does not require sophisticated electrical equipment; the result is available within 3 hours. If appropriate technical training is included, the application of this dot-blot ELISA kit in clinical laboratories throughout Thailand should be possible.


Subject(s)
Angiostrongylus cantonensis/immunology , Animals , Antibodies, Helminth/blood , Enzyme-Linked Immunosorbent Assay/methods , Humans , Immunoblotting/methods , Reproducibility of Results , Sensitivity and Specificity , Strongylida Infections/blood , Thailand
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